3种冷冻方法对猪MII期卵母细胞冷冻后微丝分布和脂肪颗粒变化的影响

doi:10.11843/j.issn.0366-6964.2014.07.010

Abstract

Abstract:

The purpose of this study was to determine the microfilament distribution，lipid droplets and their ultrastructure changes that resulted from vitrified porcine MII-stage oocytes with 3 vitrification techniques（Straw method，CLV method and OPS method）.The results showed that：(1) The cooling speed of CLV method was the highest，and got 42 639 ℃•min^-1.(2) CLV method got the highest normal distribution rate (47.06%)，and it was much higher than OPS method (38.04%) and Straw method (30.95%).(3) There was no significant differences in the normal distribution rate of lipid droplets between CLV and OPS methods after thaw，but they were much higher than that of Straw method (P<0.05).(4) Decreased content of large lipid droplets in thawed oocytes were found out after ultrastructural observation with transmission electron microscopy (TEM)，and most of lipid droplets in thawed oocytes were homogeneous and very few of them were heterogeneous.These results concluded that the lipid droplets and their distribution after vitrification were damaged greatly.Improving cooling speed could decrease the injury of microfilament，and then reduce the damage degree of distribution in lipid droplets.

CLC Number:

S814.8

WU Cai-feng，DAI Jian-jun，ZHANG Shu-shan，LI Wei-jie，XU Li，ZHANG De-fu. Effects of Three Vitrification Methods on the Microfilament Distribution and Lipid Droplets Changes of Porcine MII-stage Oocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(7): 1097-1103.

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Effects of Three Vitrification Methods on the Microfilament Distribution and Lipid Droplets Changes of Porcine MII-stage Oocytes

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